Dear Dicty people,
I might have misunderstood the explanation by Alex.
Forget everything, please.
yuzuru
> Dear Alex and Vidya,
>
> How about my idea?
>
> Inside the ecmA-lacZ mutant cells; the ecmA promoter on vector
> deprives the authentic ecmA promoter on genome of its transcription
> factors$B"*(BThe cells cannot differentiate into prestalk cells (Insead,
> the cells may differentiate into prespore cells, which produce DIF)
> $B"*(BDIF comes$B"*(BWild type cells tend to differentiate into PstO.
>
> Reporter gene systems include a fatal risk like 'Uncertainty
> Principle: Indeterminacy Principle by Heisenberg' in the field of
> Quantum mechanics.
> The proper fate of a molecule and thus of the cell might be changed
> by your observation with a reporter gene system.
>
> Best wishes,
>
> Yuzuru
>
> On 2010/05/13, at 22:08, Vidya Nanjundiah wrote:
>
>> Dear Alex,
>>
>> It isn't clear which wild type-mutant mix you have carried out. We
>> have
>> seen a preference on the part of wild type cells to occupy the collar
>> region in mixes involving the wild type and the trishanku mutant (see
>> Jaiswal et al., Differentiation, Volume 74 Issue 9-10, Pages 596 -
>> 607,
>> 2006, "Trishanku, a novel regulator of cell-type stability and
>> morphogenesis in Dictyostelium discoideum").
>>
>> As for w$(D+r(Bld type ecmA-lacZ transformants by themselves, do you mean
>> that
>> the tip does not show lacZ expression but the collar region does?
>>
>> cheers
>>
>> Vidya
>>
>>
>>> Hello everyone,
>>>
>>> I have been doing alot of studies in chimera, mainly by following
>> individual cell types using lacZ reporters. One of the defects I
>> see is
>> very strange - ecmA:lacZ (construct S) in the ecmO collar region. Even
>> more strangely is that in a mutant/wild type ecmA:lacZ mix, it is the
>> wild type cells that go there!
>>>
>>> Has anyone ever seen anything like it with things they are working
>>> on or
>> know of any papers where a similar phenotype has been seen? Its a
>> difficult search term and i'm not having much luck.
>>>
>>> Cheers
>>> Alex
>>>
>>
>>
>>
>>
>>
>> --
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>
>
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> Yuzuru Kubohara Ph.D.
> Gunma University
> Institute for Molecular & Cellular Regulation
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