Dear Oliver,

The C-terminal transmembrane region of Miro, a mitochondrial Ras-related
GTPase, will target your protein to the outer mitochondrial membrane
with the rest of the protein facing the cytosol. We have used it to
target a different protein (fused to GFP) there (Neffgen et al, Traffic
2006). Might be useful.

Regards,


Dr. Francisco Rivero
Centre for Biomedical Research
The Hull York Medical School
University of Hull
HU6 7RX Hull
Phone: +44 1482 466433
e-mail: [log in to unmask]



-----Original Message-----
From: DICTY [mailto:[log in to unmask]] On Behalf Of
Oliver Hoeller
Sent: 04 October 2010 23:47
To: [log in to unmask]
Subject: [DICTY - 421] In vivo organelle markers with cytoplasmic
portion

  Dear Dicty workers,
please let me pick your brains for the following:
I'd like to redirect cytoplasmic proteins to new places within the cell 
upon a stimulus ( e.g. using a small molecule as a hetero-dimerization 
agent). This could be achieved by having a cytoplasmic recruitee bind to

a recruiter on an organelle (ER, Golgi, Mitochondria...).
For that to work I'd like help with finding suitable recruiters. In 
other words -  markers for organelles that you or others have used 
successfully in vivo. These proteins should also contain a cytoplasmic 
portion that can tolerate fusion to be useful.

So far I have successfully used calnexin, which however failed later for

other reasons.

All hints are appreciated!

Thanks a lot,
-Oliver


-- 

o l i v e r   h o e l l e r
postdoc
lim&  weiner labs
ucsf


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