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| Date: | Mon, 2 Jun 2014 14:21:38 +0000 |
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We tried adding DNA oligos to Dicty cells (with no transfection reagent), and observed levels of the associated protein increasing; this happened with oligos that would bind to the mRNA at the 5' UTR or coding region, and for 2 different genes.
Richard Gomer
Professor of Biology
Texas A&M University
ILSB MS 3474
301 Old Main Drive
College Station, TX 77843-3474
979 458 5745
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From: DICTY [[log in to unmask]] on behalf of Maja Marinoviæ [[log in to unmask]]
Sent: Monday, June 02, 2014 8:05 AM
To: [log in to unmask]
Subject: [DICTY] question for Dicty ListServ (e-mail discussion)
Dear all,
Has anyone ever tried transient knock-down by RNA interference (siRNA)
with synthetic RNA oligos? Also have you used Lipofectamine as
transfection reagent for this purpose?
Thanks for help,
Maja Marinovic
--
Maja Marinovic, dipl. ing.
Rudjer Boskovic Institute
Division of Molecular Biology
Bijenicka 54
10 000 Zagreb
Croatia
Phone +38514571324
Fax +38514561177
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