Hi Weiye,
We've expressed histones with Cherry on them.
You get red nuclei.  No sign of the clumping that comes with TagRFP.
However, we've noticed weak expression with other fusions- much lower than
you would get with GFP.  This causes a problem as the autofluorescence
becomes a pain at these levels of expression.
So we have just Dictyfied the whole sequence, to give it a helping hand.
The plasmid arrived last week and is as yet untested.
Happy to make available.
Thanks,
Jonathan

 
Jonathan Chubb
Department of Cell and Developmental Biology and
MRC Laboratory for Molecular Cell Biology
University College London
Gower Street
London
WC1E 6BT




On 01/07/2013 15:55, "Wang, Weiye (NIH/NCI) [F]" <[log in to unmask]>
wrote:

>Hello all,
>
>Could anyone please share the experience of expressing mCherry fusion
>proteins in Dictyostelium? Does mCherry express well in Dicty?
>
>Thank you.
>
>Weiye
>
>Weiye Wang
>Laboratory of Cellular and Molecular Biology
>National Cancer Institute
>Bldg 37, Rm 2056
>37 Convent Drive
>Bethesda MD 20892
>Ph# 301-594-0768
>