Hi Sascha,
This is not normal for Dictyostelia. Seems a fixation or embedding artefact to me. There are also lighter patches over the cytosol, which do not show up that much, because the background is already lighter.

Cheers,
Pauline

________________________________________
From: DICTY [[log in to unmask]] on behalf of Sascha Thewes [[log in to unmask]]
Sent: 12 June 2013 16:12
To: [log in to unmask]
Subject: [DICTY] EM mitochondria

Dear Dicty researchers,

We did in our lab EM pictures of Dicty cells and found some strange looking
mitochondria. Mitos seem to have wholes or inclusions (see arrows in the
attached image). As it is the first time for us to make EM pictures we are
not sure if we just see preparation artefacts or something else. Has anybody
suggestions?

Thanks for your help,

Sascha.

--
Sascha Thewes, PhD
Institute for Biology - Microbiology
Department of Biology, Chemistry, Pharmacy
Freie Universität Berlin
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14195 Berlin
Germany

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