dictyNews
Electronic Edition
Volume 47, number 13
June 18, 2021
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Abstracts
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Cyclic AMP is dispensable for allorecognition in Dictyostelium cells
overexpressing PKA-C
Shigenori Hirose, Mariko Katoh-Kurasawa, and Gad Shaulsky,
Department of Molecular and Human Genetics, Baylor College of
Medicine, Houston, TX, USA
Journal of Cell Science, Accepted
Allorecognition and tissue formation are interconnected processes
that signaling between matching pairs of the polymorphic
transmembrane proteins TgrB1 and TgrC1 in Dictyostelium.
Extracellular and intracellular cAMP signaling are essential to
many developmental processes. The three adenylate cyclase
genes, acaA, acrA, and acgA, are required for aggregation,
culmination, and spore dormancy, respectively, and some of their
functions can be suppressed by activation of the cAMP-dependent
protein kinase PKA. Previous studies suggested that cAMP signaling
might be dispensable for allorecognition and tissue formation, while
others argued that it is essential throughout development. Here we
show that allorecognition and tissue formation do not require cAMP
production as long as PKA is active. We eliminated cAMP production
by deleting the three adenylate cyclases and overexpressed PKA-C
to enable aggregation. The cells exhibited cell polarization, tissue
formation, and cooperation with allotype-compatible wild-type cells,
but not with incompatible cells. Therefore, TgrB1-TgrC1 signaling
controls allorecognition and tissue formation, while cAMP is
dispensable as long as PKA-C is overexpressed.
submitted by: Gad Shaulsky[[log in to unmask]]
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