Dear all,


A professor in my department, specialized in electrophysiology and patch-clamping, agreed to give it a try and patch-clamp Dictyostelium. Although I’ve never seen this described in papers, we decided to try anyway. She thought the cells would wrap themselves around the electrode, or just run away. However, to her astonishment, Dicty cells were able to ENTER on the 1-micron-pipettes used to patch clamp. 


So, my question is: is there any trick around to stop cells and still have them in a “physiological” condition? I was thinking latrunculin, but not sure if it’s the best answer….


Cheers, Wanessa


Lab Pierre Cosson, Geneva
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