Hi Kari,

The plasmids from Annette are available in the Stock Center:
http://dictybase.org/db/cgi-bin/dictyBase/SC/plasmid_details.pl?id=17
http://dictybase.org/db/cgi-bin/dictyBase/SC/plasmid_details.pl?id=18

Best wishes,
Petra

On May 27, 2014, at 10:02 AM, Annette Müller-Taubenberger wrote:

> Dear Kari,
> 
> We used to visualize the ER in Dicty with GFP-calreticulin and calnexin-GFP. 
> See Müller-Taubenberger et al., EMBO J 20, 2001.
> 
> Best wishes,
> 
> Annette
> 
> -----Ursprüngliche Nachricht----- 
> From: Kari Naylor
> Sent: Tuesday, May 27, 2014 4:00 PM
> To: [log in to unmask]
> Subject: [DICTY] Imaging the ER
> 
> Hello all:
> 
> We are looking to do some real time imaging of the ER, and were wanting to 
> use ER Tracker (or some other dye)  Our initial experiments have shown no 
> staining and we have not been able to find examples in the literature of 
> using ER Tracker in Dicty (not that we looked real hard).  Does anyone have 
> any information on using ER Tracker or why it might not work in Dicty.
> 
> Thanks in advance
> 
> Kari
> 
> Kari Naylor PhD
> University of Central Arkansas
> Biology Department
> 139 Lewis Science Center
> ph:501-450-5826
> [log in to unmask]