Hello All,

We are trying to construct a long hairpin for RNAi using essentially the 
technique described by Rosel and Kimmel for COP9.  We have the long arm 
of the hairpin (1100 bp) in pMB38 but are having problems inserting the 
short arm (800 bp).  Does anyone have any tricks for getting what seems 
to be a difficult ligation to work? 

Thanks for any suggestions you may have for us.

Brenda Blacklock