I agree with David. We use RNeasy. It is great for yields, purity and good
for qRT-PCR and microarray also if you also use their on-column DNase kit.
Plus, unlike trizol etc. no nasty chemicals.

Mike


Wanessa Cristina DE LIMA wrote:
>  Dear all,
>
> Does anyone have suggestions for good commercially available kits for RNA
> extraction? I'm using a NucleoSpin kit from Macherey-Nagel, but yields (at
> least in my hands) are normally bad (i  use 5-10 million cells/prep).
>
> Thanks a lot for the help,
> Wanessa de Lima
> (lab Pierre Cosson, UNIGE, Geneva)
>


----------------------------------------
Michael A. Myre, PhD
Center for Human Genetic Research
Massachusetts General Hospital
Harvard Medical School
Richard B. Simches Research Center
185 Cambridge St., CPZN 5.612A
Boston, MA  02114
Ph. 617-643-5536
Fax 617-726-5735
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