Dear Dicty Researchers,

I was trying to find a publication where somebody has measured or  
estimated the cytosolic salt concentrations of prominent cations and  
anions but I was not successful. I doubt that the commonly used low  
ionic strength Soerenson phosphate buffer reflects a physiological  
situation for cytosolic proteins.  We would like to use an optimal  
physiological buffer for cytosolic proteins with regard to pH, ion  
concentration and ion composition. Any helpful hints are welcome.

All the best,

Ralph


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Prof. Dr. Ralph Gräf

University of Potsdam
Institute of Biochemistry and Biology
Department of CELL BIOLOGY

Karl-Liebknecht-Strasse 24-25, Haus 26
14476 Potsdam
Germany

Tel.: +49-(0)331 9775520
FAX: +49-(0)331 9775522

http://www.bio.uni-potsdam.de/professuren/zellbiologie/aktivitaten