dictyNews
Electronic Edition
Volume 43, number 5
March 17, 2017
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Abstracts
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Protein Kinase A regulates the Ras, Rap1, and TORC2 pathways in
response to the chemoattractant cAMP in Dictyostelium
Margarethakay Scavello, Alexandra R. Petlick, Ramya Ramesh,
Valery F. Thompson, Pouya Lotfi, and Pascale G. Charest.
Journal of Cell Science, in press
Efficient directed migration requires tight regulation of
chemoattractant signal transduction pathways in both space and
time, but the mechanisms involved in such regulation are not
well understood. Here, we investigated the role of Protein
Kinase A (PKA) in controlling signaling of the chemoattractant
cAMP in Dictyostelium. We found that cells lacking PKA display
severe chemotaxis defects, including impaired directional sensing.
Although PKA is an important regulator of developmental gene
expression, including the cAMP receptor cAR1, our studies using
exogenously expressed cAR1 in cells lacking PKA, cells lacking
adenylyl cyclase A (ACA), and cells treated with the PKA-selective
pharmacological inhibitor H89, suggest that PKA controls
chemoattractant signal transduction, in part, through the
regulation of RasG, Rap1, and TORC2. As these pathways control
the ACA-mediated production of intracellular cAMP, they lie
upstream of PKA in this chemoattractant signaling network.
Consequently, we propose that the PKA-mediated regulation of
the upstream RasG, Rap1, and TORC2 signaling pathways is part
of a negative feedback mechanism controlling chemoattractant
signal transduction during Dictyostelium chemotaxis.
submitted by: Pascale Charest [[log in to unmask]]
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