Plus any evidence that the Klebsiella we use can be treated as category 1, not category 2 (likely to cause infection and trouble)?


On 31 Aug 2011, at 21:33, <[log in to unmask]> wrote:

> The website could be used by Dicty workers to help with writing 
> applications for GMO permissions. In Germany they want lots of 
> references to substantiate that it is not hazardous. The website 
> will/can probably not (yet) serve as a reference by itself.
> 
> Some reference for (non-) contamination of mammalian cell culture and 
> for the lack of mycoplasma (if proven) could also be helpful.
> wolfgang
> 
> On Wed, 31 Aug 2011 14:48:28 -0400
>  Michael Myre <[log in to unmask]> wrote:
>> Good idea but might need something more official than a web page. I 
>> have
>> had to deal with the USDA and APHIS and acquire a permit just to be 
>> able
>> to use Formedium HL5.
>> 
>> Where my lab is, the ignorance about Dicty being non-hazardous and 
>> having
>> little to no risk of contamination to our cell culture rooms can get 
>> quite
>> tiring. Especially with mycoplasma contamination issues...most are 
>> unaware
>> that a technician is more of a risk if that tech is a carrier and
>> routinely passes their cells.
>> 
>> Wolfgang Nellen wrote:
>>>                    Dear all,
>>>     especially in Germany, safety regulations for transgenic 
>>> organisms
>>> are strict. We just encounter the request that filters in the work
>>>  benches should be inactivated by formaldehyde. It may be useful
>>> (especially for the German and probably other European workers) to
>>> have references at hand that argue against health hazards by
>>> Dictyostelium. I proposed to DictyBase to establish a "safety page".
>>>   This could be immediately used in applications for permission to
>>>  work with recombinant Dicty.
>>>     I am asking for your help to compile references for this. Below 
>>> I
>>> list references that I have already found to provide evidence for:
>>> cell inactivation at 35°C, stopp of growth at 27°C, spore
>>> inactivation at 55°C, and the long scientific work with
>>> Dictyostelium without any reported health problems for the workers.
>>>     Any further good reasons why Dicty is not hazardous are 
>>> appreciated.
>>>   I miss e.g. a reference that dispersal by wind is not possible 
>>> (or
>>>    very unlikely).
>>> 
>>>                                          Cotter, D. A. and 
>>>       K. B.
>>> Raper (1966). "Spore
>>> germination in Dictyostelium
>>> discoideum." Proc Natl Acad
>>>     Sci U S A 56(3): 880-7.
>>> Cotter, D. A. and         K. B.
>>> Raper         (1968). "Spore
>>> germination in strains of
>>> Dictyostelium         discoideum
>>> and         other members of the
>>> Dictyosteliaceae." J Bacteriol
>>> 96(5): 1690-5.     Katz, E. R.
>>> and L.         Y. Bourguignon
>>>     (1974). "The cell cycle and
>>> its relationship to aggregation
>>> in         the         cellular
>>> slime mold, Dictyostelium
>>> discoideum." Dev Biol
>>> 36(1): 82-7.     Loomis, W. F.
>>> and         S. Wheeler (1980).
>>>      "Heat shock response of
>>> Dictyostelium." Dev Biol 79(2):
>>> 399-408.     Raper, K. B.
>>> (1935).         "Dictyostelium
>>> discoideum, a new species of
>>> slime mold from         decaying
>>>        forest leaves." J.
>>>    Agr. Res. 50:
>>> 135-147.           Thanks     a
>>> lot!
>>>     wolfgang
>>>     --    Wolfgang Nellen Abt. Genetik Univ. Kassel 
>>> Heinrich-Plett-Str.
>>> 40 34132 Kassel, Germany  phone: (++49) 561 8044805 fax: (++49) 561
>>> 8044800 mobile: (++49) 17665620661 [log in to unmask]
>>> http://www.biologie.uni-kassel.de/genetics/
>>> http://www.sciencebridge.net http://www.vbio.de/
>> 
>> 
>> ----------------------------------------
>> Michael A. Myre, PhD
>> Center for Human Genetic Research
>> Massachusetts General Hospital
>> Harvard Medical School
>> Richard B. Simches Research Center
>> 185 Cambridge St., CPZN 5.612A
>> Boston, MA  02114
>> Ph. 617-643-5536
>> Fax 617-726-5735
>> [log in to unmask]
>> ----------------------------------------
>> 
>> 
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> 
> Wolfgang Nellen
> Abt. Genetik
> Univ. Kassel
> Heinrich-Plett-Str. 40
> 34132 Kassel
> Germany