DICTY Archives

March 2014, Week 1

DICTY@LISTSERV.IT.NORTHWESTERN.EDU
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Subject:
Re: DAPI staining
From:
Richard Gomer <[log in to unmask]>
Reply To:
DICTY <[log in to unmask]>
Date:
Fri, 7 Mar 2014 21:33:29 +0000
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Hi-
You will see the DAPI staining mitochondrial DNA, thus there will be background staining in the cells outside of the nucleus

Richard Gomer
Texas A&M University
ILSB    MS 3474
301 Old Main Drive
College Station, TX  77843-3474
979 458 5745

________________________________________
From: DICTY [[log in to unmask]] on behalf of Sarah Marie Bruno [[log in to unmask]]
Sent: Friday, March 07, 2014 3:07 PM
To: [log in to unmask]
Subject: [DICTY] DAPI staining

I am a student working with Dr. Carl Franck at Cornell University. I have been trying to stain the nuclei of the dicty cells with DAPI, but I have observed a lot of nonspecific binding. Does anyone have any tips or protocol for selectively staining the nuclei using DAPI?

Thank you!

Sarah Marie Bruno

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