Hi Sarah,
you can try to use topro 3 (1:1000) it is much better dye.

good luck,

Hellen



> I am a student working with Dr. Carl Franck at Cornell University. I have
> been trying to stain the nuclei of the dicty cells with DAPI, but I have
> observed a lot of nonspecific binding. Does anyone have any tips or
> protocol for selectively staining the nuclei using DAPI?
>
> Thank you!
>
> Sarah Marie Bruno
>


-- 
Dr. Hellen Ishikawa-Ankerhold
Institute for Anatomy and Cell Biology
Ludwig Maximilian University of Munich
Schillerstr. 42
80336 Muenchen, Germany
Ph:  +49 89-218075-873 (office)
Ph:  +49 89-218075-862 (lab)
Fax: +49 89-218075-004

http://www.zellbio.anatomie.med.uni-muenchen.de/

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