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January 2010, Week 1

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Subject:
phosphate-free FM
From:
Changhoon Choi <[log in to unmask]>
Reply To:
DICTY <[log in to unmask]>
Date:
Thu, 7 Jan 2010 12:37:04 -0800
Content-Type:
text/plain
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text/plain (19 lines) 
Hi all,

I am planning to do metabolic labeling to detect phosphorylation of  
some dicty proteins. I found a nice protocol for 32P labeling on the  
dictybase web site, but the problem is phosphate-free FM medium. Our  
lab is small and doesn't have all chemicals required for FM medium. As  
far as I know, all commercial FM media contain potassium phosphate. Is  
there anybody that knows any way to get phosphate-free FM medium or  
can anybody share some stock solutions with us?

Thanks and happy new year!!

Changhoon

Changhoon Choi, PhD
University of California, San Francisco
513 Parnassus Ave, HSW618
San Francisco, CA 94143

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