dictyNews

Electronic Edition

Volume 49, number 20

July 28, 2023



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Abstracts

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Fluctuations of formin binding in the generation of membrane 

patterns



Mary Ecke, Jana Prassler, and Günther Gerisch*



Max Planck Institute of Biochemistry, D-82152 Martinsried, 

Germany

*Corresponding author: [log in to unmask]





Biophysical Journal, 

https://doi.org/10.1016/j.bpj.2023.07.014



Abstract

Circular actin waves that propagate on the substrate-attached 

membrane of Dictyostelium cells separate two distinct membrane 

domains from each other: an inner territory rich in 

phosphatidyl-(3,4,5) trisphosphate (PIP3), and an external area 

decorated with the PIP3-degrading 3-phosphatase PTEN. During 

wave propagation, the inner territory increases on the expense 

of the external area. Beyond a size limit, the inner territory 

becomes unstable, breaking into an inner and an external domain. 

The sharp boundary between these domains is demarcated by the 

insertion of an actin wave. During the conversion of inner 

territory to external area, the state of the membrane fluctuates, 

as visualized by dynamic landscapes of Formin B binding. Here we 

analyze the Formin B fluctuations in relation to three markers 

of the membrane state: activated Ras, PIP3, and PTEN.





Submitted by Mary Ecke ([log in to unmask])

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[End dictyNews, volume 49, number 20]