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February 2018, Week 1

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Subject:
Re: Reverse Transcriptase for low RNA concentration
From:
Richard Gomer <[log in to unmask]>
Reply To:
DICTY <[log in to unmask]>
Date:
Thu, 1 Feb 2018 18:26:20 +0000
Content-Type:
text/plain
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text/plain (26 lines) 
Hi-
We normally use 300 - 1000 ng/ microliter for RT-PCR, going lower should not be that much of a problem, and we use
Maxima H Minus Reverse Transcriptase from Thermo Fisher
best,
Richard Gomer
Department of Biology
Texas A&M University
301 Old Main Drive
College Station, TX  77843-3474
979 458 5745
________________________________________
From: DICTY [[log in to unmask]] on behalf of Otmane Lamrabet [[log in to unmask]]
Sent: Thursday, February 1, 2018 9:59 AM
To: [log in to unmask]
Subject: [DICTY] Reverse Transcriptase for low RNA concentration

Dear All,

Does anyone have already used the SuperScript™ II Reverse Transcriptase for RT-PCR experiments from total extracted RNAs of dictyostelium discoideum?
Which Reverse Transcriptase could you recommend to me if I have a low concentration of total extracted RNAs (less than 100ng/microl)?

Thanks in advance for your reply.

Best,

Otmane

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