Before using Formedium we consistently used the same peptone (e.g. #3 or #2) for an experiment. Changing the peptone seemed to effect the wild clones in particular.
Gerda
On Jan 31, 2013, at 10:27 AM, Joan Strassmann wrote:
> Generally switching media can be risky, particularly in the middle of an experiment. We have increasingly turned to the very uniform product from Formedium.
> Joan
> On Jan 31, 2013, at 8:04 AM, John B. Biggins wrote:
>
>> Dicty newbie here:
>>
>> Anyone see any difference in using bacto peptone instead of proteose peptone in SM plates?
>>
>> Thanks
>
> _________________________________
> Joan E. Strassmann
> Professor of Biology
>
> Department of Biology
> Washington University in St. Louis
> One Brookings Drive
> Campus Box 1137
> St. Louis MO 63130
>
> phone: (314) 935-3527
> fax: (314) 935-4432
> cell: (832) 978-5961
> skype: strassm
> e-mail [log in to unmask]
> http://strassmannandquellerlab.wordpress.com/
>
> Blogs: http://sociobiology.wordpress.com/
> http://slowbirding.wordpress.com/
> http://goodbyehouston.wordpress.com/
> Twitter: @JoanStrassmann
>