Agree with the GenePulser XCell from Bio-Rad. Unlike some other equipment
from Bio-Rad, it actually seems to be built to last. The Harvard Apparatus
one seems even more sturdy on the pictures, but I've never used that. I
would at least recommend to go with one with square wave possibilities.
Browsing parameter space for optimisation (such as dye loading) (and when
using different cell strains!) is so much easier with square wave compared
to exp. decay.

Cheers,

Douwe


> Dear members of the DictyList,
>
> We are currently investigating our options regarding our electroporator:
> maintenance or purchase ?
> While knowing that potentially damaged parts are not manufactured anymore
> for our model...
>
> [current one: GenePulser II from Bio-Rad]
>
> Which one have proven efficient and reliable in your hands ? or do you
> have any recommandations on some to avoid for working with Dicty ?
>
> Thanks in advance for your answers,
> Best Regards,
>
> Romain
>
>
> Romain Bodinier
> Centre Médical Universitaire
> Dpt of Cell Physiology and Metabolism
> 1 rue Michel Servet
> CH 1211 Geneva 4, Switzerland
>
> Tel. (41) 22 379 5294
> Fax (41) 22 379 5338
>