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March 2009, Week 1

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Subject:
Re: Electroporation in 0.2 cm cuvette
From:
Robert Gundersen <[log in to unmask]>
Reply To:
DICTY <[log in to unmask]>
Date:
Wed, 4 Mar 2009 15:12:24 -0500
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We routinely perform electroporation in 0.2 cm cuvettes in an older BioRad Gene Pulser.  The settings are 1.2 kV, 100 ohms and 3 uF.  A time constant of 0.5 or 0.6 gives virtually 100% success; 0.4 is around 50% and less than 0.4 does not yield any
transformants.   We always keep cells chilled for 10 after electroporation.

Bob Gundersen

Chair & Associate Professor
Biochemistry, Microbiology and Molecular Biology
5735 Hitchner Hall
University of Maine
Orono, ME 04469-5735
(207) 581-2802

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