The Cellometer Mini or T4 from Nexcelom are great, easy and fast. The 
cost of the mini I am not sure about but the T4 is ~ 4K.

The Cellometer Vision is ~ 35K...and fantastic. You can count cell 
number, concentration, diameter, viability (in live and dead cells) in 
less than a minute, GFP, RFP and YFP expression analysis of 
transformants, proliferation, autophagy, apoptosis, cell cycle, surface 
markers etc. can all be performed.

Richard's method sounds easy and affordable too. The only drawbacks on 
cell counting is that it doesn't take into consideration total 
biomass...which is important when analyzing mutants that differ in size. 
Comparing cell strains (RNAs, metabolites etc.) by using equal cell 
numbers can introduce a lot of variability if biomass isn't accounted 
for.

Best,

Mike



On 2013-01-21 15:07, Damer, Cynthia Kay wrote:
> Dear Colleagues,
>
> I am interested in buying an automated cell counter.  Does anyone
> have any advice about what kind to buy for use with Dicty cells?
>
> Thanks!
>
> Cynthia
>
> Cynthia K. Damer
> Professor
> Biology Department
> Brooks Hall Room 229
> Central Michigan University
> Mount Pleasant, MI 48859
> (989) 774-3455


----------------------------------
Michael A. Myre, PhD
Center for Human Genetic Research
Massachusetts General Hospital
Harvard Medical School
Richard B. Simches Research Center
185 Cambridge St., CPZN 5.612A
Boston, MA  02114
Ph. 617-643-5536
Fax 617-726-5735
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