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March 2017, Week 2

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Subject:
Bacterial streak Dicty growth assay
From:
"Knecht, David" <[log in to unmask]>
Reply To:
DICTY <[log in to unmask]>
Date:
Tue, 14 Mar 2017 19:39:42 +0000
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I have a vague recollection of a paper where someone used a streak assay to compare rates of consumption of different types of bacteria by Dictyosteleids.  My mental image is an agar plate with several linear streaks of bacteria.  The Dicty were inoculated at one end and the rate at which they moved up and cleared the line used as a measure of Dicty growth.  Perhaps it was a way to compare whether they consumed that type of bacteria at all.   Can anyone point me toward an assay like that?  It sounds simple, but I suspect there are tricks.  Thanks- Dave

Dr. David Knecht
Professor , Department of Molecular and Cell Biology 
University of Connecticut	
91 N. Eagleville Rd.
U-3125
Storrs, CT 06269-3125
860-486-2200

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