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February 2009, Week 2

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Subject:	Re: electroporator
From:	Masashi Fukuzawa <[log in to unmask]>
Reply To:	DICTY <[log in to unmask]>
Date:	Thu, 12 Feb 2009 09:16:26 +0900
Content-Type:	text/plain
Parts/Attachments:	text/plain (29 lines)

Hi

We are quite unhappy with our BioRad Micropulser (a cheaper option). After a
bunch of testing (cuvette gap, number of pulses, kV, etc) we concluded that
it does not work for Dicty. There is a Dicty transformation protocol
provided but it always gave zero to a few colonies.

BioRad could not explain the reason and gave us an advice "please do not use
it for slime mold!"

I am wondering if there is somebody successfully applied Micropulser to
Dicty transformation.

Masashi

-- 
Masashi Fukuzawa, PhD

Department of Biology
Faculty of Agriculture and Life Science
Hirosaki University
3 Bunkyo-cho, Hirosaki
Aomori 036-8561
Japan

> We are quite happy with our Bio-Rad electroporator. Using 1 mm cuvettes at
> 0.85 kV/25 uF (2 pulses with 5 seconds break between pulses) we get nice
> results.

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