Dear Dr. Fukuzawa and all,
May I spare some anti-cofilin antibody? Also, by any chance, does anybody know who currently have cofilin knocked out dicty cells? Here is the reference article I got from dictybase:
http://www.ncbi.nlm.nih.gov/pubmed?cmd=search&term=11683919
Many thanks and best wishes,
Xuehua. 

Xuehua Xu, PhD
Staff scientist
LIG/NIAID/NIH

-----Original Message-----
From: Masashi Fukuzawa [mailto:[log in to unmask]] 
Sent: Sunday, June 02, 2013 7:49 PM
To: [log in to unmask]
Subject: Re: [DICTY] anti-cofilin Abs

Dear Mike,

I have excavated the antibody from the National Heritage in the top shelf of my deep freezer. It was from Dr Sameshima who received the antibody from Dr Aizawa a decade ago.

The writing on the box reads "anti-coffilin3 C terminal peptide". It is most likely rabbit polyclonal but no information of activity, purification, etc.
The solution is whitish so it should have gone through some purification.

The total volume of the stock is about 6 ml, please let me know if you are interested and how much volume you may need. I will not use them by my retirement and it might be better to move the stock where it is needed.

--
Masashi Fukuzawa, PhD

Department of Biology
Faculty of Agriculture and Life Science
Hirosaki University
3 Bunkyo-cho, Hirosaki
Aomori 036-8561
Japan

Tel: +81 (0)172 36 2111 ext 2596
[log in to unmask]




> I was curious if anyone has an antibody to Dd cofilin and if they 
> could
spare
> a small aliquot for IF. The literature suggests there is one
available from
> either Dr. Aizawa or Dr. Yahara but I have yet to find
their correspondence
> emails. Thanks.

Mike