dictyNews
Electronic Edition
Volume 48, number 24
December 2, 2022
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Abstracts
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C2GAP2 is a Common Regulator of Ras Signaling for Chemotaxis,
Phagocytosis, and Macropinocytosis
Xuehua Xu1*, Henderikus Pots2, Bernd K. Gilsbach3, Dustin Parsons1,
Douwe M. Veltman2, Sharmila G. Ramachandra1, Haoran Li1,
Arjan Kortholt2, and Tian Jin1
1Chemotaxis Signaling Section, Laboratory of Immunogenetics, National
Institute of Allergy and Infectious Diseases, National Institutes of
Health, 5625 Fishers Lane, 4N03, Rockville, MD 20852, USA.
2 Department of Cell Biochemistry, University of Groningen,
Nijenborgh 7, 9747 AG Groningen, The Netherlands.
3German Center for Neurodegenerative Diseases (DZNE),
Otfried-Müller-Str. 23, D-72076 Tübingen, Germany.
* To whom correspondence may be addressed.
Email: [log in to unmask]
Frontiers in Immunology, in press
Phagocytosis, macropinocytosis, and G protein coupled receptor-mediated
chemotaxis are Ras-regulated and actin-driven processes. The common
regulator for Ras activity in these three processes remains unknown.
Here, we show that C2GAP2, a Ras GTPase activating protein, highly
expressed in the vegetative growth state in model organism Dictyostelium.
C2GAP2 localizes at the leading edge of chemotaxing cells, phagosomes
during phagocytosis, and macropinosomes during micropinocytosis. c2gapB-
cells lacking C2GAP2 displayed increased Ras activation upon folic acid
stimulation and subsequent impaired chemotaxis in the folic acid
gradient. In addition, c2gaB- cells have elevated phagocytosis and
macropinocytosis, which subsequently results in fastercell growth.
C2GAP2 binds multiple phospholipids on the plasmamembrane and the
membrane recruitment of C2GAP2 requires calcium. Taken together,we
show a shared negative regulator of Ras signaling that mediatesRas
signaling for chemotaxis, phagocytosis, and macropinocytosis.
Submitted by Xuehua Xu [[log in to unmask]]
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