Salts: Maeda years ago
pH: about 7.4 from in vivo NMR Kay et al, 1986.
Rob

Sent from my iPhone

On 24 Jun 2010, at 17:38, Richard Gomer <[log in to unmask]>  
wrote:

> Dunno about specific ions, we measured osmolality of lysed cells (see
> our 2004 JBC AlrA paper), its ~ 250 mOsm/ kg H2O for veg cells, and is
> 400 at 6 hours.
>
> For cytosolic pH, see Aerts et al 1985 Cell.  We saw cytosolic pH's in
> veg cells ranging from 6.4 to 7.4 depending on cell cycle phase (see  
> our
> 2000 JBC RtoA paper)
>
> Also, be careful with 'Sorenson's phosphate buffer', about 15 years  
> ago
> I read a protocol using this  buffer, and didn't have a recipe- I
> emailed the Dicty listserver and got ~6 replies.  Each recipe was
> different.....
>
> cheers
> Richard Gomer
>
>
> Richard Gomer
> Professor
> TAMU Biology
> ILSB    MS 3474
> 301 Old Main Drive
> College Station, TX  77843-3474
> 979 458 5745
>
>
>
>
>>>> Ralph Gräf <[log in to unmask]> 06/24/10 6:54 AM >>>
> Dear Dicty Researchers,
>
> I was trying to find a publication where somebody has measured or
> estimated the cytosolic salt concentrations of prominent cations and
> anions but I was not successful. I doubt that the commonly used low
> ionic strength Soerenson phosphate buffer reflects a physiological
> situation for cytosolic proteins.  We would like to use an optimal
> physiological buffer for cytosolic proteins with regard to pH, ion
> concentration and ion composition. Any helpful hints are welcome.
>
> All the best,
>
> Ralph
>
>
> ---------------------------------------------------------
>
> Prof. Dr. Ralph Gräf
>
> University of Potsdam
> Institute of Biochemistry and Biology
> Department of CELL BIOLOGY
>
> Karl-Liebknecht-Strasse 24-25, Haus 26
> 14476 Potsdam
> Germany
>
> Tel.: +49-(0)331 9775520
> FAX: +49-(0)331 9775522
>
> http://www.bio.uni-potsdam.de/professuren/zellbiologie/aktivitaten