Douwe generated C and N terminal SNAP vectors (pDM 1301 and 1302) as extrachromosomal and also he made integrating versions (1303 and 1304). There are mCherry and LimE versions as well. I am not sure who from Rob Kay’s lab has the complete set but I sure hope they are somewhere. I only have the database file and I am not sure it is complete. Dave Dr. David Knecht Professor, Department of Molecular and Cell Biology University of Connecticut 91 N. Eagleville Rd. U-3125 Storrs, CT 06269-3125 860-486-2200 > On Mar 28, 2019, at 12:25 PM, Ralph Gräf <[log in to unmask]> wrote: > > Dear Dicty-colleagues, > > has anybody successfully tried the SNAP- or CLIP-tags in Dictyostelium and has generated suitable cloning vectors? > > Many thanks in advance, Ralph > > -- > _________________________________________ > > Prof. Dr. Ralph Gräf > University of Potsdam > Institute for Biochemistry and Biology > Karl-Liebknecht-Str. 24-25, Haus 26 > 14476 Potsdam > Germany > > Tel: +49 331 9775520 > https://urldefense.proofpoint.com/v2/url?u=https-3A__nam01.safelinks.protection.outlook.com_-3Furl-3Dhttps-253A-252F-252Furldefense.proofpoint.com-252Fv2-252Furl-253Fu-253Dhttps-2D3A-5F-5Fwww.uni-2D2Dpotsdam.de-5Fibb-2D2Dzellbiologie-5Findex.html-2526d-253DDwIDaQ-2526c-253DyHlS04HhBraes5BQ9ueu5zKhE7rtNXt-5Fd012z2PA6ws-2526r-253DG0i-2DxkKvWepiOT01FF-5FNx9XkaEEFt5Dttsc3yIePxBPU44aHfHsMfVSlUygwIJiN-2526m-253DSU6Vz8YdcbrHg9a2BEiMQDwdAR1jzluyEezo7BcoLb8-2526s-253D-2DiPxaQrBSiB-2Dlq3UGOBeXNOkiTOHFLvcS9lUM5Q0otg-2526e-26amp-3Bdata-3D02-257C01-257Cdavid.knecht-2540UCONN.EDU-257C13761e14f1f84887085408d6b3a67871-257C17f1a87e2a254eaab9df9d439034b080-257C0-257C1-257C636893924917854613-26amp-3Bsdata-3DrwlJ9FM-252BnPqx-252FFBsCPP3OqPiWr9HWIeoowHwqbgbmqg-253D-26amp-3Breserved-3D0-3D&d=DwIGaQ&c=yHlS04HhBraes5BQ9ueu5zKhE7rtNXt_d012z2PA6ws&r=G0i-xkKvWepiOT01FF_Nx9XkaEEFt5Dttsc3yIePxBPU44aHfHsMfVSlUygwIJiN&m=TWqmpMDjp9QVPUc0HT5jDRUf9EnU4PoPYs-QPX_kzGM&s=1nDzyjr0IPZLbF5OKaH5SK7AGxLFE3tflnftSLV87Vo&e= >
