Sorry everyone, I guess it's Friday!

Happy holidays!

Petra

On Dec 10, 2010, at 4:03 PM, Petra Fey wrote:

> Hi Maria,
> 
> By mistake I sent the response to you already to the ListServ, and it got already a couple of responses, pasted below. If you have general Dicty questions you should subscribe, as often important topics are being discussed.
> 
> Best wishes,
> Petra
> 
> ----
> I ran into this problem earlier this year.  I consulted composition tables published by BD for all of their peptones, and found that Proteose Peptone No. 3 is most similar to Thiotone E peptone.  Thus, I now make HL5 with a 50:50 mixture of Proteose Peptone No. 2 (#212120) and Proteose Peptone No. 3 (#211693).  Cells seem pretty happy in this medium.  I have also had good luck with the pre-made stuff from Formedium, but it's difficult to get because of customs issues.
> 
> Good luck!
> 
> Dan Dickinson
> Stanford University
> ----
> 
> We have started using proteose peptone 3 in stead of proteose peptone 2 from BD over a year ago when proteose peptone 2 was on a too long backorder, and have not noticed any differences in growth. We now use only proteose peptone 3 in our HL5.
> 
> Greetings,
> 
> Hanke
> ----
> On Dec 10, 2010, at 10:54 AM, Petra Fey wrote:
> 
> Dear Maria,
> 
> This is a question that's best suited for the ListServ, as it should be of interest to the whole Dicty community. To write to the  Dicty Listserv, send your email to [log in to unmask]
> 
> To receive the responses to the ListServ, please subscribe by filling out this form:
> http://dictybase.org/db/cgi-bin/dictyBase/colleague/colleagueUpdate
> 
> Good luck in your endeavor, but I'm sure there are people out there who are facing (or faced) the same problem, and sooner or later it concerns many.
> 
> Best wishes,
> Petra
> 
> On Dec 10, 2010, at 10:22 AM, Maria Abad wrote:
> 
>> Hello,
>> 
>> Thiotone E peptone (BBL # 212302) has been discontinued. Could you recommend  a substitute product to make HL5  medium?