dictyNews
Electronic Edition
Volume 48, number 19
September 24, 2022
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Abstracts
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Evolving social behavior through selection of single-cell adhesion in
Dictyostelium discoideum
Sandrine Adiba1,3, Mathieu Forget1,2 and Silvia De Monte1,2
1 Institut de Biologie de l’ENS (IBENS), Département de biologie, Ecole
normale supérieure, CNRS, INSERM, Université PSL, 75005 Paris, France
2 Department of Evolutionary Theory, Max Planck Institute for
Evolutionary Biology, Plön, Germany
3 Institut de Biologie de l’ENS (IBENS), Département de
biologie, Ecole normale supérieure, CNRS, INSERM, Université PSL, 75005
Paris, France
Corresponding author:
Sandrine Adiba
iScience, accepted
doi: 10.1016/j.isci.2022.105006
The social amoeba Dictyostelium discoideum commonly forms chimeric
fruiting bodies. Genetic variants that produce a higher proportion of
spores are predicted to undercut multicellular organization unless
cooperators assort positively. Cell adhesion is considered a primary
factor driving such assortment, but evolution of adhesion has not been
experimentally connected to changes in social performance. We modified
by experimental evolution the efficiency of individual cells in
attaching to a surface. Surprisingly, evolution appears to have produced
social cooperators irrespective of whether stronger or weaker adhesion
was selected. Quantification of reproductive success, cell-cell
adhesion, and developmental patterns, however, revealed two distinct
social behaviors, as captured when the classical metric for social
success is generalized by considering clonal spore production. Our work
shows that cell mechanical interactions can constrain the evolution of
development and sociality in chimeras and that elucidation of proximate
mechanisms is necessary to understand the ultimate emergence of
multicellular organization.
Submitted by Sandrine Adiba [[log in to unmask]]
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A novel gene, Le-Dd10, is involved in fruiting body formation of
Lentinula edodes
A. Kishikawa1, S. Hamada1, I. Kamei1, Y. Fujimoto1, K. Miyazaki 2,
M. Yoshida 1,3
1 Department of Agricultural Science, Kinki University, Nakamachi
3327-204, Nara 631-8505, Japan
2 Kyushu Research Center, Forest Products Research Institute,
Kurokami 4-11-16, Kumamoto 860-0862, Japan
3 Osaka University of Comprehensive Children Education, Yusato 6-4-26,
Higashisumiyoshi-ku, Osaka 546-0013, Japan
Corresponding author:
M. Yoshida
Archives of Microbiology in press
The cDNA library prepared from Lentinula edodes, Hokken 600 (H600),
primordia was screened by using cDNA expressed specifically in
Dictyostelium discoideum prestalk as a probe. Twenty-one clones,
Le-Dd1~21, were isolated from the L. edodes primordia cDNA library.
Functional analysis of each gene was carried out by transformation
into protoplast cells from L. edodes Mori 252.
(M252) mycelia with the overexpression vector pLG-RasF1 of each gene
because M252 protoplast cells were transformed with eleven-fold higher
efficiency than H600 cells. Transformants with the overexpression vector
of Le-Dd10 formed a fruiting body at almost the same time as H600, a
positive control, although M252, a negative control, did not form a
fruiting body under culture conditions. This suggested that Le-Dd10 is
involved in the formation of fruiting bodies. Single-strand conformation
polymorphism analysis revealed that Le-Dd10 is located on No. 4 linkage
group of L. edodes. The properties of Le-Dd10 products were investigated
by Western blotting analysis using polyclonal antibodies against
GST:Le-Dd10 fusion proteins. As a result, 56-kDa, 27-kDa, and 14-kDa
protein bands appeared in primordial and fruiting body stages, although
the expected molecular weight of the Le-Dd10 product was 50 kDa.
Submitted by M. Yoshida [[log in to unmask]]
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