Perhaps misled by a background in bacterial genetics before becoming a
Dicty person, I had always assumed that the Susman's called it HL based on
Hershey Broth (HB) and Luria-Bretani (LB) Broth that might have been a
starting point for the formulation of what finally worked for growing
Dicty.

It definitely did not come from Harvey Lodish, although Harvey's lab did
much later contribute a method for freezing Dicty at -80C that was easier
than using the "Bull Straws" in an earlier protocal from Susman or
Ashworth.

Best to all,
Daphne

> In my earlier work on cell growth attached here, 2 papers were cited.
> Those are the pioneer works on axenic strains.
>
> Cocucci and Susman simply mentioned "liquid medium referred to hereafter a
> HL-5 and contains the following : glucose (16 mg/ml) ; proteose peptone
> (14 mg/ml) ; yeast extract (7 mg/ml) ; Na2HP04 .7H20 (0.95 mg/ml) ; KH
> 2PO4 (0.5 mg/ml)" . There are no indications about "H, L, and 5".
>
> Watts and Ashworth used almost the same liquid medium but not named it.
>
> Does anybody know about those pioneers?
>
>
> Masazumi Sameshima
>   ----- Original Message -----
>   From: Knecht, David
>   To: [log in to unmask]
>   Sent: Thursday, January 23, 2014 5:34 AM
>   Subject: [DICTY] Dicty history lesson
>
>
>   I am in Dicty intro teaching mode and wondered if anyone knew who coined
> the term HL5 (the first reference I have found so far called it HL/5)
> and what the HL stands for?  Bonus points for inaccurate but humorous
> answers.  Cheers- Dave
>
>
>   David Knecht, Ph.D.
>   Professor and Head of Core Microscopy Facility
>   Department of Molecular and Cell Biology
>   U-3125
>   91 N. Eagleville Rd.
>   University of Connecticut
>   Storrs, CT 06269
>   860-486-2200
>   860-486-4331 (fax)
>
>
>
>
>
>
>
>
>