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December 2016, Week 4

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Subject:
Re: Gene control for qRT-PCR
From:
Sascha Thewes <[log in to unmask]>
Reply To:
DICTY <[log in to unmask]>
Date:
Tue, 27 Dec 2016 13:22:54 +0100
Content-Type:
text/plain
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text/plain (17 lines) 
Hello Mona,

you can use gpdA. We also use it and it is widely accepted (see for
example Marquez-Lopez et al., 2016, Dev Genes Evol. 226(1):27-35). An
alternative would be the mitochondrial ancoded rnlA.

Good luck,

Sascha.

> Hello everybody,
> I'm planning to do qRT-PCR for some genes in dicty, I would ask you which
> gene you advise me to use as gene control? can I use for example GAPDH
> (gpdA, Dictybase entry DDB0185087)? there are other genes normally used?
> thank you and have a good end of the year..
>

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