Funny enough, we were just making plans for constructing an APEX2 that is targeted to the luminal side of the ER membrane. By having a signal sequence at the N-terminus, we hope that it is codon-optimized enough to be translated properly.

Kind regards, Markus Maniak


-----Ursprüngliche Nachricht-----
Von: DICTY <[log in to unmask]> Im Auftrag von Ryan Gawryluk
Gesendet: Freitag, 5. Juli 2019 17:29
An: [log in to unmask]
Betreff: Re: [DICTY] Inquiry APEX2 Dicty

Hi Lyudmil,

I’m also hoping to use APEX. I don’t think anyone has reported using it, though I expect codon usage will be important, as it was for BioID.

Ryan

> On Jul 5, 2019, at 08:11, Lyudmil Raykov <[log in to unmask]> wrote:
> 
> Dear all,
> 
> I’d like to use the engineered peroxidase APEX2 in D. discoideum in order to seek for potential proximal partners of my protein of interest.
> 
> Therefore, I have two questions:
> 
> 1. Has anyone used APEX2 in D. discoideum cells?
> 
> 2. Does anyone know, whether codon bias correction is necessary for the usage of APEX2 in D. discoideum?
> 
> Thanking you for your time.
> 
> Kind regards,
> 
> Lyudmil Raykov