dictyNews
Electronic Edition
Volume 48, number 6
March 25, 2022
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Abstracts
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Confirmation and variability of the Allee effect in Dictyostelium
discoideum cell populations, possible role of chemical signaling
within cell clusters
Igor Segota, Matthew M. Edwards, Arthur Campello, Brendan H. Rappazzo,
Xiaoning Wang, Ariana Strandburg-Peshkin , Xiao-Qiao Zhou , Archana
Rachakonda, Kayvon Daie, Alexander Lussenhop, Sungsu Lee , Kevin
Tharratt , Amrish Deshmukh, Elisabeth M. Sebesta, Myron Zhang,
Sharon Lau, Sarah Bennedsen, Jared Ginsberg, Timothy Campbell,
Chenzheng Wang, and Carl Franck
Physical Biology 19 (2022) 026002
https://doi.org/10.1088/1478-3975/ac4613
In studies of the unicellular eukaryote Dictyostelium discoideum, many have
anecdotally observed that cell dilution below a certain ‘threshold density’
causes cells to undergo a period of slow growth (lag). However, little is
documented about the slow growth phase and the reason for different
growth dynamics below and above this threshold density. In this paper, we
extend and correct our earlier work to report an extensive set of experiments,
including the use of new cell counting technology, that set this slow-to-fast
growth transition on a much firmer biological basis. We show that dilution
below a certain density (around 1E4 cells / ml) causes cells to grow slower
on average and exhibit a large degree of variability: sometimes a sample
does not lag at all, while sometimes it takes many moderate density cell
cycle times to recover back to fast growth. We perform conditioned media
experiments to demonstrate that a chemical signal mediates this
endogenous phenomenon. Finally, we argue that while simple models
involving fluid transport of signal molecules or cluster-based signaling explain
typical behavior, they do not capture the high degree of variability between
samples but nevertheless favor an intra-cluster mechanism.
Submitted by Carl Franck [[log in to unmask]]
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