Dear Dicty researchers, has anyone successfully employed the halo-tag to label structures inside living cells? Are there any experiences which halo-tag ligands are properly taken up by Dicty amoebae? In the only published example I could find labeling occurred on the extracellular side. We would be grateful for helpful hints or references to publications. All the best, Ralph -- __________________________________ Prof. Dr. Ralph Gräf Dept. Cell Biology University of Potsdam Karl-Liebknecht-Str. 24-25, Haus 26 14476 Potsdam Germany Tel: +49 (0)331 9775520 https://urldefense.com/v3/__https://www.uni-potsdam.de/en/ibb-zellbiologie/index__;!!Dq0X2DkFhyF93HkjWTBQKhk!S9M3x57REzhVsnePunLm03aeGVjExcOBpTtTvIlnZ_tETHVTgJSgeZc5yaDymgLFZLXLBwZ7EZJq30A6khW7W23rz2hoADFp6Ks$