Hello,

To answer your second question, addition of DIF-1 to starved cells would kill (as tested by non-regrowth in HL5) 50% of :
-  wild type cells, within 8-10h, of developmental vacuolar cell death
-  atg1- mutant cells, within 2.5h, of necrotic cell death.
 
With best regards,

Pierre

________________________________________________
Pierre Golstein
Centre d'Immunologie de Marseille-Luminy
INSERM/CNRS/Univ.Medit.
Case 906, Campus de Luminy, Avenue de Luminy
13288 Marseille cedex 9, France
 tel : 33 (0)4 91 26 94 68
Fax : 33 (0)4 91 26 94 30
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http://www.ciml.univ-mrs.fr/
________________________________________
De : DICTY [[log in to unmask]] de la part de Monica Hagedorn [[log in to unmask]]
Date d'envoi : mercredi 13 juin 2012 17:59
À : [log in to unmask]
Objet : [DICTY - 794] To kill Dicty

Hello,
I am very sorry but we are looking for something that upon expression in Dicty would kill the axenic Dicty-cells quickly.

Does anyone have the Dicty expression plasmid for RicinA or cholera toxin and would be willing to provide us with a tiny bit?
Is there something that would induce quick necrotic or autophagic death?

I would be happy for any input,
Greetings from Hamburg,
Monica



Dr. Monica Hagedorn
Bernhard-Nocht-Institute for Tropical Medicine
Bernhard-Nocht-Strasse 74
20359 Hamburg
Germany
Phone: +49 (0)40-42818 541
Fax:  +49 (0)40-42818 512

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