Using fura2 fluorescence, Baskar et al. found that cellular calcium levels
were around 100nM in slugs formed by NC4 cells of D. discoideum (with
variations depending on where the cells came from; see Int J Dev Biol
44(5), 491-498, 2000,
http://www.ijdb.ehu.es/web/contents.php?vol=44&issue=5).

Vidyanand Nanjundiah

> Dear Dicty Researchers,
>
> I was trying to find a publication where somebody has measured or
> estimated the cytosolic salt concentrations of prominent cations and
> anions but I was not successful. I doubt that the commonly used low
> ionic strength Soerenson phosphate buffer reflects a physiological
> situation for cytosolic proteins.  We would like to use an optimal
> physiological buffer for cytosolic proteins with regard to pH, ion
> concentration and ion composition. Any helpful hints are welcome.
>
> All the best,
>
> Ralph
>
>
> ---------------------------------------------------------
>
> Prof. Dr. Ralph Gräf
>
> University of Potsdam
> Institute of Biochemistry and Biology
> Department of CELL BIOLOGY
>
> Karl-Liebknecht-Strasse 24-25, Haus 26
> 14476 Potsdam
> Germany
>
> Tel.: +49-(0)331 9775520
> FAX: +49-(0)331 9775522
>
> http://www.bio.uni-potsdam.de/professuren/zellbiologie/aktivitaten
>
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