George, your guess was correct, it was a non-dicty contaminant - I worked in that lab designing some electronics for them (talking to them about what they were doing with their plates and test tubes was the thing that got me interested in biology)
Richard Gomer
Thomas Powell '62 Professor of Biology
Texas A&M University
ILSB MS 3474
301 Old Main Drive
College Station, TX 77843-3474
979 458 5745
________________________________________
From: DICTY [[log in to unmask]] on behalf of George McNamara [[log in to unmask]]
Sent: Wednesday, February 01, 2017 11:17 AM
To: [log in to unmask]
Subject: Re: [DICTY] Dicty handedness
Hi David,
No study LEFT behind ... Here you go:
J Cell Sci. 1980 Jun;43:341-66.
P2: a behavioural mutant of Dictyostelium discoideum.
Frantz CE.
Abstract
P2 is a mutant strain isolated from the cellular slime mould,
Dictyostelium discoideum strain NC-4. P2 differs from NC-4 in 3 major
respects. P2 lacks the cyclic AMP chemotactic and signalling properties
and the aggregative development of NC-4. P2 development consists of slow
differentiation of resistant microcysts, which have not previously been
reported in D. discoideum. Most important, P2 amoebae display a novel
pattern of movement, quite distinct from that of NC-4 amoebae and any
other related amoebae for which data are available. P2 amoebae move on
agar at a mean velocity of 13.3 micrometer/min, almost twice as fast as
NC-4 amoebae. P2 amoebae have a persistence time, or directional memory
'half-life', of 13.2 min, over 2.5 times the NC-4 value. However, this
measure is based on straight-line movement, and actual P2 resistence may
be much greater because P2 amoebae move almost always in gradual left
turns and left loops interspersed with occasional long, straight
segments. When P2 amoebae are plated in drops on agar, they migrate away
in all directions, and the expanding drop edge can move at nearly the
mean individual cell velocity. Spiral bands of amoebae can be seen in
the expanding area of the drop; these invariably unwind from the
original drop in a counterclockwise direction. The persistent
left-turning behaviour of individual P2 amoebae is probably the major
cause of this pattern of movement from drops. It also probably helps
explain the similar coordinated banding behaviour observed in fields of
cells. Amoebae move long distances in bands of contacting or separated
cells, with no apparent organizing centre. Statistical analysis shows
that P2 and amoebae have some tendency to cluster, and cell-cell contact
interactions may be strong enough to promote band formation.
PMID: 7419624
https://urldefense.proofpoint.com/v2/url?u=http-3A__jcs.biologists.org_content_43_1_341.long&d=CwIDaQ&c=yHlS04HhBraes5BQ9ueu5zKhE7rtNXt_d012z2PA6ws&r=G0i-xkKvWepiOT01FF_Nx9XkaEEFt5Dttsc3yIePxBPU44aHfHsMfVSlUygwIJiN&m=ZVOFoYOf6qOomyAXQseOSkWGIPEi-RJzO7BpZJKhLsI&s=jDRmhQQQ2C_OO1e8_W9n5ComAhmFLmmGQkua6lOECew&e=
//
My guess (even as a grad student) was that it was some protozoan
contamination and that "revertant(s)" were wild-type Dicty that
contaminated the contaminants cultures (though the text only mentions
"partial revertants").
If anyone is connected with J Cell Science and can post the reviews
online, that would be great (hopefully any 'statute of limitations' has
expired).
George
p.s. my PubMed searches were worth my time - turned up a 1975 article on
iontophoresis of cyclic AMP from C&R.
https://urldefense.proofpoint.com/v2/url?u=http-3A__www.cell.com_biophysj_pdf_S0006-2D3495-2875-2985852-2D8.pdf&d=CwIDaQ&c=yHlS04HhBraes5BQ9ueu5zKhE7rtNXt_d012z2PA6ws&r=G0i-xkKvWepiOT01FF_Nx9XkaEEFt5Dttsc3yIePxBPU44aHfHsMfVSlUygwIJiN&m=ZVOFoYOf6qOomyAXQseOSkWGIPEi-RJzO7BpZJKhLsI&s=5KV99ZOhF2P6XemU7dTxwKYEMMkwvqEHaNu54tTzaa0&e=
On 2/1/2017 10:52 AM, Knecht, David wrote:
> I was asked by a colleague if Dicty had any handedness. I haven’t been able to find any literature, but I thought of aggregation, and I have a few examples where some movies show spiral arms and aggregation mounds rotating clockwise and some counter-clockwise, but they are from different experiments. Is there data as to whether there is a preferred directionality, and if so, is there a hypothesis as to what controls directionality? Dave
>
> Dr. David Knecht
> Professor
> Department of Molecular and Cell Biology U-3125
> University of Connecticut
> 91 N. Eagleville Rd.
> Storrs, CT 06269-3125
> 860-486-2200
>
--
George McNamara, PhD
Houston, TX 77054
[log in to unmask]
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