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February 2019, Week 3

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Subject:
LIC/Gibson assembly
From:
Hanke van der Wel <[log in to unmask]>
Reply To:
DICTY <[log in to unmask]>
Date:
Mon, 18 Feb 2019 21:43:27 +0000
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I tried using the LIC method to ligate 3 fragments into a KpnI-linearized vector using the HiFi DNA Assembly Master Mix from NEB, but was unsuccesful. 
No clones on the plate after transfection, and no ligated fragments detected with pcr.

Has anyone tried this method with Dicty DNA (or maybe another form of Gibson assembly), and if so, do you have hints/suggestions?

Thanks!



Hanke van der Wel
Research Professional IV
Biochemistry & Molecular Biology
University of Georgia
120 E. Green Street
Life Sciences Building,  A308A
Athens, GA 30602
706/542-8859

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