dictyNews
Electronic Edition
Volume 49, number 17
June 25 2023
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Abstracts
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Derivatives of Differentiation-Inducing Factor 1 Differentially
Control Chemotaxis and Stalk Cell Differentiation in
Dictyostelium discoideum.
Hidekazu Kuwayama 1, Haruhisa Kikuchi 2 and Yuzuru Kubohara 3
1 Graduate School of Life and Environmental Sciences, University
of Tsukuba, Tsukuba 305-8572, Japan
2 Division of Natural Medicines, Faculty of Pharmacy, Keio
University, Tokyo 105-8512, Japan
3 Laboratory of Health and Life Science, Graduate School of
Health and Sports Science, Juntendo University, Inzai 270-1695,
Japan
Biology, published
Differentiation-inducing factors 1 and 2 (DIF-1 and DIF-2)
are small lipophilic signal molecules that induce stalk cell
differentiation but differentially modulate chemotaxis toward
cAMP in the cellular slime mold Dictyostelium discoideum;
DIF-1 suppresses chemotactic cell movement in shallow cAMP
gradients, whereas DIF-2 promotes it. The receptor(s) for DIF-1
and DIF-2 have not yet been identified. We examined the effects
of nine derivatives of DIF-1 on chemotactic cell movement toward
cAMP and compared their chemotaxis-modulating activity and
stalk cell differentiation–inducing activity in wild-type and mutant
strains. The DIF derivatives differentially affected chemotaxis
and stalk cell differentiation; for example, TM-DIF-1 suppressed
chemotaxis and showed poor stalk-inducing activity, DIF-1(3M)
suppressed chemotaxis and showed strong stalk-inducing activity,
and TH-DIF-1 promoted chemotaxis. These results suggest that
DIF-1 and DIF-2 have at least three receptors: one for stalk
cell induction and two for chemotaxis modulation. In addition,
our results show that the DIF derivatives can be used to
analyze the DIF-signaling pathways in D. discoideum.
Submitted by Yuzuru Kubohara [[log in to unmask]]
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