Dear Jon,

If memory serves me right, Wurster and Kurzenberger were able to monitor
light scattering oscillations in a beaker containing starved Dd cells in
suspension. Oxygen was bubbled into the suspension through a baffle.
Unfortunately I don't have the paper on hand right now to confirm this.
The reference is "Periodic cell aggregation in suspensions of
Dictyostelium discoideum", Differentiation 41(1): 1–4, 1989.

Regards,

Vidya

_________________________
>>From: DICTY [[log in to unmask]] on behalf of Jon Moore
>>[[log in to unmask]]
>>Sent: Saturday, February 20, 2016 10:21 AM
>>To: [log in to unmask]
>>Subject: [DICTY] Anyone ever bubble instead of shake?
>>
>>Hi.
>>
>>There is a bit of a long and uninteresting story behind this one,
>>but has anyone ever bubbled their dicty cultures for agitation
>>instead of shaking?  If so, were there any complications?
>>
>>Peace,
>>
>>Jon Moore
>>Assistant Professor of Biology
>> & Lab Coordinator
>>Pomona College
>>Claremont, California 91711
>