The issue is primarily one of reproducibility. The feeder bacteria can probably grow in the new medium using bacto peptone, but you won't have the privilege of all prior optimizations using standard SM. Experiments will have to be reoptimized from that point on.
On Jan 31, 2013, at 10:04 AM, "John B. Biggins" <[log in to unmask]> wrote:
> Dicty newbie here:
>
> Anyone see any difference in using bacto peptone instead of proteose peptone in SM plates?
>
> Thanks