Dear Emily,

I think Tom Williams pretty much answered your question in his paper on
the physiological regulation of macropinocytosis: Journal of Cell Science
2018 131: jcs213736 doi: 10.1242/jcs.213736
Look at FigS3 in particular.

Tom is nearby in Dundee, should you ever go that way.

Rob

> Hi Emily- My lab has done a variety of phagocytosis experiments.  I
> don’t have a clear answer to your question since we never directly
> compared bacterially grown and axenic cells for phagocytosis rate.  What I
> can tell you is that axenically grown cells phagocytose very efficiently
> and I would be surprised if there was a major difference.  We routinely
> used axenically grown cells for uptake of both latex beads and fluorescent
> bacteria (Maselli et al. 2002).  Dave
>
> Dr. David Knecht
> Professor, Department of Molecular and Cell Biology
> University of Connecticut
> 91 N. Eagleville Rd.
> U-3125
> Storrs, CT 06269-3125
>
>
>
> On Dec 17, 2020, at 7:21 AM, Emily Addington
> <[log in to unmask]<mailto:[log in to unmask]>>
> wrote:
>
>
> *Message sent from a system outside of UConn.*
>
>
> Hello all! I’d like to pose what is perhaps a rather naïve question to
> all you seasoned Dictyostelium experts. Are there any studies on how
> phagocytic rate of Dictyostelium amoeba differs in suspension with axenic
> and non-axenic medium? For instance, do Dictyostelium grown alongside
> bacteria in axenic medium phagocytose less or at a slower rate than
> Dictyostelium grown in Sorensen’s or KK2 buffer alongside bacteria? I am
> interested in axenic rather than non-axenic Dictyostelium strains. I’d
> be very grateful for any information on this!
>
> Emily
>
>