dictyNews
Electronic Edition
Volume 45, number 16
June 28, 2019
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Abstracts
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Halogen-Substituted Derivatives of Dictyostelium Differentiation-Inducing
Factor-1 Suppress Serum-Induced Cell Migration of Human Breast
Cancer MDA-MB-231 Cells in Vitro
Kyoko Totsuka 1, Yuka Makioka 1, Kyoichi Iizumi 2, Katsunori Takahashi 3,
Yoshiteru Oshima 4, Haruhisa Kikuchi 4,*, Yuzuru Kubohara 1,2,*
1 Laboratory of Health and Life Science, Graduate School of Health and
Sports Science, Juntendo University, Inzai, Chiba 270-1695, Japan
2 Laboratory of Health and Life Science, Department of Health Science,
Faculty of Health and Sports Science, Juntendo University, Inzai, Chiba
270-1695, Japan
3 Department of Medical Technology, Faculty of Health Science, Gunma
Paz College, Takasaki 370-0006, Japan
4 Laboratory of Natural Product Chemistry, Graduate School of
Pharmaceutical Sciences, Tohoku University, Aoba-ku, Sendai 980-8578,
Japan
* Corresponding authors
Biomolecules, in press
Abstract: Triple-negative breast cancer (TNBC) is highly proliferative and
metastatic, and because it lacks three major molecular targets for
chemotherapy (estrogen receptor, progesterone receptor, and human
epidermal receptor 2), it is extremely refractory. Differentiation-inducing
factor 1 (DIF-1) and DIF-3, which are chlorinated alkylphenones, are lead
anticancer compounds found in the cellular slime mold Dictyostelium
discoideum. Here, we examined the in vitro effects of DIF-1, DIF-3, and
25 DIF derivatives on cell proliferation and serum-induced cell migration
in human MDA-MB-231 cells, a model TNBC cell line. We found that
Br-DIF-1, a chlorine-to-bromine-substituted derivative of DIF-1, strongly
suppressed cell migration (IC50, 3.8 microM) with negligible effects on
cell proliferation (IC50, >20 microM). We then synthesized 18 derivatives
of Br-DIF-1 and examined the in vitro effects of these derivatives on cell
proliferation and serum-induced cell migration in MDA-MB-231 cells.
Among the derivatives, Br-DIF-1(+1), Br-DIF-1(+2), and Br-DIF-3(+2)
exhibited strong anti-cell migration activities with IC50 values of 1.5, 1.0,
and 3.1 microM, respectively, without affecting cell proliferation
(IC50, >20 microM). These results suggest that these Br-DIF derivatives
are good lead compounds for the development of anti-metastatic drugs
against TNBC.
submitted by: Yuzuru Kubohara [[log in to unmask]]
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The AJ514 antibody recognizes the common antigen 1 from Dictyostelium
discoideum by immunofluorescence
Wanessa Cristina Lima, Pierre Cosson
Antibody Reports, 2019, vol. 2, e45
https://oap.unige.ch/journals/abrep/article/view/45
The AJ514 antibody, derived from the 221-342-5 hybridoma, detects by
immunofluorescence the common antigen 1 from Dictyostelium discoideum.
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The AJ520 antibody recognizes the Dictyostelium vacuolar H+-ATPase
subunit A by immunofluorescence
Wanessa Cristina Lima, Pierre Cosson
Antibody Reports, 2019, vol. 2, e46
https://oap.unige.ch/journals/abrep/article/view/46/
The AJ520 antibody, derived from the 221-35-2 hybridoma, detects by
immunofluorescence the full-length vacuolar H+-ATPase subunit A from
Dictyostelium discoideum.
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The AJ520 antibody recognizes the Dictyostelium vacuolar H+-ATPase
subunit A by Western blot
Cyril Guilhen, Wanessa Cristina Lima
Antibody Reports, 2019, vol. 2, e47
https://oap.unige.ch/journals/abrep/article/view/47/
The AJ520 antibody, derived from the 221-35-2 hybridoma, detects by
Western blot the full-length vacuolar H+-ATPase subunit A from
Dictyostelium discoideum.
submitted by: Wanessa Lima [[log in to unmask]]
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[End dictyNews, volume 45, number 16]
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