The website could be used by Dicty workers to help with writing
applications for GMO permissions. In Germany they want lots of
references to substantiate that it is not hazardous. The website
will/can probably not (yet) serve as a reference by itself.
Some reference for (non-) contamination of mammalian cell culture and
for the lack of mycoplasma (if proven) could also be helpful.
wolfgang
On Wed, 31 Aug 2011 14:48:28 -0400
Michael Myre <[log in to unmask]> wrote:
> Good idea but might need something more official than a web page. I
>have
> had to deal with the USDA and APHIS and acquire a permit just to be
>able
> to use Formedium HL5.
>
> Where my lab is, the ignorance about Dicty being non-hazardous and
>having
> little to no risk of contamination to our cell culture rooms can get
>quite
> tiring. Especially with mycoplasma contamination issues...most are
>unaware
> that a technician is more of a risk if that tech is a carrier and
> routinely passes their cells.
>
> Wolfgang Nellen wrote:
>> Dear all,
>> especially in Germany, safety regulations for transgenic
>>organisms
>> are strict. We just encounter the request that filters in the work
>> benches should be inactivated by formaldehyde. It may be useful
>> (especially for the German and probably other European workers) to
>> have references at hand that argue against health hazards by
>> Dictyostelium. I proposed to DictyBase to establish a "safety page".
>> This could be immediately used in applications for permission to
>> work with recombinant Dicty.
>> I am asking for your help to compile references for this. Below
>>I
>> list references that I have already found to provide evidence for:
>> cell inactivation at 35°C, stopp of growth at 27°C, spore
>> inactivation at 55°C, and the long scientific work with
>> Dictyostelium without any reported health problems for the workers.
>> Any further good reasons why Dicty is not hazardous are
>>appreciated.
>> I miss e.g. a reference that dispersal by wind is not possible
>>(or
>> very unlikely).
>>
>> Cotter, D. A. and
>> K. B.
>> Raper (1966). "Spore
>> germination in Dictyostelium
>> discoideum." Proc Natl Acad
>> Sci U S A 56(3): 880-7.
>> Cotter, D. A. and K. B.
>> Raper (1968). "Spore
>> germination in strains of
>> Dictyostelium discoideum
>> and other members of the
>> Dictyosteliaceae." J Bacteriol
>> 96(5): 1690-5. Katz, E. R.
>> and L. Y. Bourguignon
>> (1974). "The cell cycle and
>> its relationship to aggregation
>> in the cellular
>> slime mold, Dictyostelium
>> discoideum." Dev Biol
>> 36(1): 82-7. Loomis, W. F.
>> and S. Wheeler (1980).
>> "Heat shock response of
>> Dictyostelium." Dev Biol 79(2):
>> 399-408. Raper, K. B.
>> (1935). "Dictyostelium
>> discoideum, a new species of
>> slime mold from decaying
>> forest leaves." J.
>> Agr. Res. 50:
>> 135-147. Thanks a
>> lot!
>> wolfgang
>> -- Wolfgang Nellen Abt. Genetik Univ. Kassel
>>Heinrich-Plett-Str.
>> 40 34132 Kassel, Germany phone: (++49) 561 8044805 fax: (++49) 561
>> 8044800 mobile: (++49) 17665620661 [log in to unmask]
>> http://www.biologie.uni-kassel.de/genetics/
>> http://www.sciencebridge.net http://www.vbio.de/
>
>
> ----------------------------------------
> Michael A. Myre, PhD
> Center for Human Genetic Research
> Massachusetts General Hospital
> Harvard Medical School
> Richard B. Simches Research Center
> 185 Cambridge St., CPZN 5.612A
> Boston, MA 02114
> Ph. 617-643-5536
>Fax 617-726-5735
> [log in to unmask]
> ----------------------------------------
>
>
>
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>whom it is
> addressed. If you believe this e-mail was sent to you in error and
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> contains patient information, please contact the Partners Compliance
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Wolfgang Nellen
Abt. Genetik
Univ. Kassel
Heinrich-Plett-Str. 40
34132 Kassel
Germany
|
