DICTY Archives

May 2014, Week 4

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Subject:
Re: Imaging the ER
From:
Annette Müller-Taubenberger <[log in to unmask]>
Reply To:
DICTY <[log in to unmask]>
Date:
Tue, 27 May 2014 17:02:52 +0200
Content-Type:
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Dear Kari,

We used to visualize the ER in Dicty with GFP-calreticulin and calnexin-GFP. 
See Müller-Taubenberger et al., EMBO J 20, 2001.

Best wishes,

Annette

-----Ursprüngliche Nachricht----- 
From: Kari Naylor
Sent: Tuesday, May 27, 2014 4:00 PM
To: [log in to unmask]
Subject: [DICTY] Imaging the ER

Hello all:

We are looking to do some real time imaging of the ER, and were wanting to 
use ER Tracker (or some other dye)  Our initial experiments have shown no 
staining and we have not been able to find examples in the literature of 
using ER Tracker in Dicty (not that we looked real hard).  Does anyone have 
any information on using ER Tracker or why it might not work in Dicty.

Thanks in advance

Kari

Kari Naylor PhD
University of Central Arkansas
Biology Department
139 Lewis Science Center
ph:501-450-5826
[log in to unmask]

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