dictyNews
Electronic Edition
Volume 33, number 9
October 9, 2009

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=========
Abstracts
=========


Dynamics of the Dictyostelium discoideum mitochondrial proteome during
vegetative growth, starvation and early stages of development


Malgorzata Czarna 1,2,§, Gregory Mathy 1, Allan Mac’Cord 1, Rowan  
Dobson 3,
Wieslawa Jarmuszkiewicz 2, Claudine M. Sluse-Goffart 1, Pierre  
Leprince 4,
Edwin De Pauw 3 and Francis E. Sluse 1

1 Laboratory of Bioenergetics and Cellular Physiology, University of  
Liege, Belgium
2 Laboratory of Bioenergetics, Adam Mickiewicz University, Poznan,  
Poland
3 Laboratory of Mass Spectrometry, University of Liege, Belgium
4 GIGA-Neuroscience, University of Liege, Belgium
§ Present address: Institute of Microbiology, Free University of  
Berlin, Germany,
E-mail: [log in to unmask]


Proteomics, in press

In this study a quantitative comparative proteomics approach has been  
used to
analyze the D. discoideum mitochondrial proteome variations during  
vegetative
growth, starvation and the early stages of development. Application of  
2D-DIGE
technology allowed the detection of around 2000 protein spots on each
two-dimensional gel with 180 proteins exhibiting significant changes  
in their
expression level. In total, 96 proteins (51 unique and 45 redundant)  
were
unambiguously identified. We show that the D. discoideum mitochondrial
proteome adaptations mainly affect energy metabolism enzymes (the Krebs
cycle, anaplerotic pathways, the oxidative phosphorylation system and
energy dissipation), proteins involved in developmental and signalling
processes as well as in protein biosynthesis and fate. The most striking
observations were the opposite regulation of expression of citrate  
synthase
and aconitase and the very large variation in the expression of the  
alternative
oxidase (AOX) that highlighted the importance of citrate and AOX in the
physiology of the development of D. discoideum. Mitochondrial energy  
states
measured in vivo with MitoTracker Orange CMTMRos showed an increase
in mitochondrial membrane polarisation during D. discoideum starvation  
and
starvation-induced development.


Submitted by Malgorzata Czarna [[log in to unmask]]
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[End dictyNews, volume 33, number 9]