dictyNews
Electronic Edition
Volume 46, number 11
April 10, 2020
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Abstracts
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Cell and molecular transitions during efficient dedifferentiation
John ME Nichols, Vlatka Antolovic, Jacob D Reich, Sophie Brameyer,
Peggy Paschke, Jonathan R Chubb
eLife 2020;9:e55435 DOI: 10.7554/eLife.55435
Dedifferentiation is a critical response to tissue damage, yet is not well
understood, even at a basic phenomenological level. Developing
Dictyostelium cells undergo highly efficient dedifferentiation, completed
by most cells within 24 hours. We use this rapid response to investigate
the control features of dedifferentiation, combining single cell imaging
with high temporal resolution transcriptomics. Gene expression during
dedifferentiation was predominantly a simple reversal of developmental
changes, with expression changes not following this pattern primarily
associated with ribosome biogenesis. Mutation of genes induced early
in dedifferentiation did not strongly perturb the reversal of development.
This apparent robustness may arise from adaptability of cells: the
relative temporal ordering of cell and molecular events was not absolute,
suggesting cell programmes reach the same end using different
mechanisms. In addition, although cells start from different fates, they
rapidly converged on a single expression trajectory. These regulatory
features may contribute to dedifferentiation responses during
regeneration.
submitted by: Jonathan Chubb [[log in to unmask]]
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A recombinant antibody toolbox for Dictyostelium discoideum
Wanessa C. Lima, Philippe Hammel and Pierre Cosson
BMC Research Notes 2020 13:206
https://bmcresnotes.biomedcentral.com/articles/10.1186/s13104-020-05048-8
The amoeba Dictyostelium discoideum has been a valuable model organism
to study numerous facets of eukaryotic cell biology, such as cell motility, cell
adhesion, macropinocytosis and phagocytosis, host–pathogen interactions
and multicellular development. However, the relative small size of the
Dictyostelium community hampers the production and distribution of reagents
and tools, such as antibodies, by commercial vendors. For the past 5 years,
our laboratory has worked to promote an increased use of recombinant
antibodies (rAbs) by academic laboratories. Here we report our efforts to
ensure that Dictyostelium researchers have access to rAbs. Using hybridoma
sequencing and phage display techniques, we generated a panel of
recombinant antibodies against D. discoideum antigens, providing a useful
and reliable set of reagents for labelling and characterization of proteins and
subcellular compartments in D. discoideum, accessible to the entire
Dictyostelium community.
submitted by: Wanessa Lima [[log in to unmask]]
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