cAMP ELISA kits utilize cAMP antibody instead of cAMP binding protein 
from bovine skeletal muscle. The problem is cAMP antibody cross-react 
with cAMP analogue like dcAMP which usually used for cAMP response assay.
ELISA kit works fine, unless samples contain any dcAMP.

The following paper may help you to see the nature of cAMP binding 
protein and the composition of the assay reagents in Amersham cAMP (3H) 
assay kit.

Clin Chim Acta. 1974 Nov 8;56(3):221-34.
A simple direct assay for cyclic AMP in plasma and other biological 
samples using an improved competitive protein binding technique.
Tovey KC, Oldham KG, Whelan JA.

Best wishes,

Kuwa

(2010/04/23 4:40), Miranda, Edward Roshan wrote:
> Hi
>
> Can someone recommend a good kit to measure intra and extracellular cAMP levels in Dicty during development?
> We used to use Amersham (now GE healthcare) cAMP (3H) assay kit and it has been discontinued and so is a kit working on simillar principles from Invitrogen. I am not sure if I can use ELISA kits or any other kits for quantitation of cAMP. Also can you comment on any modifications to the procedure of making and storing of cAMP in case you are using the kit you are recommending?
>
> Thanks in advance for your response and time.
> Roshan
>
>
>    


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