Ranjani,
I used GAPDH primers flanking the intron.Here are the primers:
GAPDH For:  GGTTGTCCCAATTGGTATTAATGG
GAPDH Rev: CCGTGGGTTGAATCATATTTGAAC
Without intron you will get 156 bp amplicon and with intron 247bp.
Best,
Sandhya.


Dr. Sandhya N Baviskar
Assistant Professor
Department of Biological Sciences
University of Arkansas- Fort Smith
5210 Grand Ave.
Fort Smith AR 72913
Phone : 479-788-7789
________________________________________
From: DICTY [[log in to unmask]] On Behalf Of Ranjani Dhakshinamoorthy [[log in to unmask]]
Sent: Monday, October 25, 2010 5:51 PM
To: [log in to unmask]
Subject: [DICTY - 432] Internal control for RT-PCR

Dear all,
       Can some one suggest me a good control for RT-PCR (real time).  If
possible the primer sequence as well. I read about IG7 and cinD
genes in the Dicty list server.  Which would be the best?

Thanks in advance!
Ranjani

--
Ranjani Dhakshinamoorthy,
Doctoral Student,
Christian-Albrechts University of Kiel,
Germany.